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1X TE buffer solution consists of 10 mM Tris, adjusted to pH 7.0 or 7.5 or 8.0 with HCl, 1 mM EDTA. These are sterile and filtered through a 0.2 μm filter. All Gene Link TE Buffers are provided nuclease-free, ready for use, and require no extra preparation. Alkalinity and free cations have been implicated in catalyzing the hydrolysis of RNA. TE Buffer pH 7.0 reduces base hydrolysis through chelation of divalent cations by EDTA and through resistance to pH changes by the Tris buffer.
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