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Lumisol™ II hybridization solution can be used for all stringent types of nucleic acid blot hybridization conditions, particularly using non-radioactive digoxigenin labeled probes. Lumisol™ II hybridization solution can be used for prehybridization and hybridization. The hybridization temperature should be calculated for proper results. Duration of hybridization can be reduced to 6 hours and overnight hybridization can be used for high sensitivity requirements and for convenience.
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