This product has been discontinued. Please call 1-800-GENE-LINK for more information.
This product contains primer pair for amplification of the SCA2 CAG triple region. Specialized amplification conditions are required to amplify large triple repeat regions. Special amplification conditions and the use of PCR additives are required to amplify large CAG triple repeat region. PCR additives are available for purchase from Gene Link.
This product should be used by researchers who would like to develop their own amplification reaction conditions.
Gene Link has optimized kit for SCA2 CAG triple repeat amplification and detection by conventional agarose or fluorescent methods and Southern blot analysis of large CAG repeats and confirmation of homozygous alleles.
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Certificate of Analysis & Product Specifications
The SCA2 Genemer™ supplied has been validated to amplify the CAG triple repeat spanning region ATXN2 gene.
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Genemer™ are optimized primer pairs for amplification of the gene fragment of interest, particularly those spanning a mutation. A wide range is available from single base pair
mutation sites, the complex triple repeat disorders and various pathogens. Genemer™ kits are complete, easy-to-use systems for reliable genotyping of large repeats in certain triple
repeat disorder.
The Genemer™ product contains 10 nmols of the primer pair that is sufficient for 400 regular 50 µl amplifications. The Genemer™ Kit contains reagents as well as control DNA sufficient for 100 amplifications.
